Table of Contents
Biol 568 Advanced Topics in Molecular Genetics
Initiation of Transcription -Euk.
Overview
Overview
General Factors
Upstream Factors
Inducible Factors
Fig 21.1 Overview of control regions for a eukaryotic gene
Transcription Control Regions
RNA Pol II
RNA Pol II
Fig 21.2 Yeast RNA Pol II subunits
Promoter Analysis
Promoter Analysis
Oocyte system
Transfection Systems
Transgenic Systems
Fig 21.3 Promoter Analysis (deletion)
Definition of promoter boundaries
Promoter Functional Analyses
Initiation of Transcription -Euk.
RNA Pol II promoter
RNA Pol II promoter
RNA Pol II promoter
Fig. 21.10 RNA pol II promoter
RNA Pol II basal apparatus
RNA Pol II Initiation
Fig 21.11 Positioning RNA Pol II
Fig 20.9 TBP bound to DNA
Fig 21.13 TBP bound to DNA
TBP binds to DNA
Add discussion about histone interactions?
Fig 21.15 Additional TFIIs bind
Fig 20.12 TF II B Interactions
Fig 20.12 TF II B Interactions
PPT Slide
TFIIB interacts with RNA pol II
Fig 21.15 Additional TFIIs bind
TFIIF
RNA pol II holocomplex
TATA-less promoters
Comparison with txn in prokaryotes
TFIID
Txn; Promoter Clearance
Fig 21.17 Release of RNA Pol II from promoter
Release of RNA Pol II from promoter
Phosphorylation of CTD
Initiation of Transcription -Euk.
Promoter Analysis
Fig 21.21 Saturation mutagenesis of a b-globin promoter
Modularity of Eukaryotic Promoters
Modularity of Eukaryotic Promoters
Fig 21.22 Promoters contain different combinations of elements
Element - TF interactions
Element - TF interactions
Element - TF interactions
Element - TF interactions
Role of Enhancers
Fig 20.19 Enhancer mutational analysis
Enhancers
What is the function of the enhancer?
Initiation of Transcription -Euk.
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