Table of Contents
Biol 568 Advanced Topics in Molecular Genetics
Ch.22 Activating Transcription
Eukaryotic Transcription
Control of gene expression
Activating txn
Response Elements
Response Elements
Response Elements
Fig 22.11 Regulatory region of a human metallothionein gene
Eukaryotic Transcription
Transcription Factor Structure/Function
Fig 22.3 Different domains for DNA binding and activating
TF Structure / Function
Gal 4 functional regions
Fig 24.4 Txn activation by Gal 4
Fig 22.5 Txn activation by tat
TF Structure/Function
Two hybrid system
The two- hybrid system
Fig 22.7 Role of co-activators
Fig 22.8 Activators & co-activators
Role of coactivators
Activators and coactivators
How does the activator stimulate txn?
RNA Pol binds as an holoenzyme
Transcription factors regulate gene expresssion
Eukaryotic Transcription
TF Structure / Function
Zinc finger TFs
Cys2-His2 Zn finger
Fig 22.13 Three Zn fingers of TF SP1
Fig 22.14 DNA binding by Zn fingers
Steroid receptor Zn fingers
Fig 22.17-18 Steroid receptor Zn fingers
Cys6 Zn Finger
GATA-1 Like Zn Finger
GATA-1 Like Zn Finger
Steroid Hormones
Steroid Hormones
Steroid Hormones
Fig 21.6 Steroid Hormone Structures
Steroid hormones
Fig 22.16 Organization of steroid receptors
Fig 22.19 Function of glucocorticoid receptors
Steroid Hormone Receptor TFs
Homodimer forming steroid receptor TFs
Heterodimer forming steroid receptor TFs
Heterodimer forming steroid receptor TFs
Steroid receptors act as dimers
Fig 22.22 Txn activation by Steroid Receptor TFs
Fig 22.22 Txn activation by Steroid Receptor TFs
TF Structure / Function
Helix-Turn-Helix (Homeodomain) TFs
Helix-Turn-Helix (Homeodomain) TFs
Fig 22.23 Homeodomain TFs
Fig 22.24 Conserved nature of homeodomain region
Fig 22.25 DNA binding by Homeodomain
TF Structure / Function
Helix-Loop-Helix TFs
Helix-Loop-Helix TFs
Fig. 22.26 Helix-Loop-Helix TFs
Helix-Loop-Helix TFs
Helix-Loop-Helix TFs
Helix-Loop-Helix TFs
Helix-Loop-Helix TFs
Helix-Loop-Helix TFs
Helix-Loop-Helix TFs
Fig 22.27
Helix-Loop-Helix TFs
TF Structure / Function
Leucine Zipper
Leucine Zipper
Fig 22.28 Leucine Zipper
Leucine Zipper
TF Structure / Function
Activation Domains
Activation Domains
Activation Domains
Activation Domains
TF Structure / Function
Eukaryotic Transcription
How can activity of TFs be regulated?
Fig 22.12 Control of activity of TFs
Fig 22.12 Control of activity of TFs
Eukaryotic Transcription
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