Table of Contents
Biol 568 Advanced Topics in Molecular Genetics
Chapter 9: Transcription
Fig 9.1 Overview of Transcription
Overview of Transcription
Basic Questions Regarding Transcription
Transcription occurs by base pairing of unpaired DNA
Fig 9.4 General nature of transcription
Fig 9.5 Transcription ěbubbleî
Stages of Transcription
Fig. 9.6 Three stages of transcription
Bacterial RNA Pol structure
Phosphodiester bond formation
Phosphodiester bond formation
Chapter 9: Transcription
Fig 9.16 Eubacterial RNA Pol subunits
Eubacterial RNA Pol Subunits
RNA Pol Functions
Other RNA Pol of importance
RNA Pol Binding
Fig 9.19 RNA Pol binding
Fig 9.19 RNA Pol binding
Fig 9.20 RNA Pol bound to promoter
Fig 9.20 RNA Pol bound to promoter
Fig 9.11 RNA Pol bound to promoter
RNA Pol in bacterial cell
Fig 9.12 RNA Pol distribution in cell
How does RNA Pol find promoters?
How does RNA Pol find promoters?
Fig 9.23 Random diffusion to promoter
Fig 9.24 Random displacement
Fig 9.25 Sliding along DNA to promoter
Promoter recognition
Consensus Sequences
Fig 9.27 Typical promoter
Analysis of proteins binding to DNA
Principle of DNA Footprinting
Fig 9.29 DNA Footprinting
Fig 9.29 Footprinting
Fig 9.30 RNA Pol binding to promoter
DNA Supercoiling
Fig 9.31 Supercoils generated in transcription
Chapter 9: Transcription
Two modes of termination in bacteria
Fig 9.27 Intrinsic Terminators
Rho dependent terminators
Fig 9.48 Rho-dependent terminator
Fig 9.49 Model for Rho action
Fig 9.49 Model for Rho action
Chapter 9: Transcription
Alternative Sigma Factors
Sigma Factors control Initiation
Alternative Sigma Factors
Alternative Sigma Factors
Fig 9.33 E. coli Sigma Factors
Alternative Sigma Factors
Alternative Sigma Factors
Alternative Sigma Factors
Fig 9.36 Conserved regions of s70
Fig 9.37 s70 binding to -10 region
Alternative s Factors in B.subtilis
B. subtilis s factors
B. subtilis s factors
B. subtilis s factors
Fig 9.41 Txn of phage SPO1
Phage SPO1 Transcription Cascade
Sporulation s factor cascade
Sporulation s factor cascade
PPT Slide
Fig 9.44 s factor cascade in sporulation
Fig 9.45 s factor cascade in sporulation Criss-cross of regulation coordinates timing
Chapter 9:Transcription
Biol 568 Advanced Topics in Molecular Genetics
Chapter 10: The Operon
Trans and Cis-acting Elements
Negative and Positive Regulation
Fig 10.2 Coordinate control of structural genes - Negative control
Negative Control
Fig 10.1 Coordinate control of structural genes - Positive control
Positive Control
Chapter 10: The Operon
Regulation of Operons
Fig 10.3 Lac Operon
Fig 10.3 Lac Operon
Regulation of lac Operon
Fig 10.5 Repressor and RNA Pol binding sites
Lac Repressor
Control of Repressor Activity
Lac Operon as Model
Chapter 10: The Operon
Fig 10.6 Lac Operon Induction
Repressor Activity
Modulating Repressor Activity
Fig 10.7 Lac operon regulation
Fig 10.8 Lac operon regulation
Regulation of Operons
Regulation of Operons
Mutational Analysis
Mutational Analysis
Mutations in the Operator
Fig 10.9 Oc Mutation
Oc Mutation
Lac I- Mutation
Lac I- Mutation
Other Lac I- Mutations
Dominant Negatives
Other Lac I- Mutations
Structure of operator
Repressor Action
Fig 10.14 Repressor-Inducer binding Two Models:
Fig 10.15 Lac repressor structure
Fig 10.16 Lac repressor structure
PPT Slide
Fig 10.18 Inducer changes repressor structure
Locations of mutations in lactose repressor
Chapter 10: The Operon
Repressor binds to operators
Repressor binds to operators
Repressor binds to looped DNA
Repressor interacts with RNA Pol
Repressor interacts with RNA Pol
Repressor is always bound to DNA
Repressor is always bound to DNA
Lac repressor binding
Induction changes repressor distribution
Chapter 10: The Operon
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